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Questions related from Michał Żorniak
Does anyone has some suggestions or experience on stimulation of gallbladder emptying in mouse in vivo? CCK-physiological doses could be an option, as we want to avoid pancreatitis induciton in...
15 April 2020 7,021 0 View
Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??
05 March 2020 5,801 3 View
Hi everyone, I am struggling with visualisation of possible deposition of cholesterol crystals in tissues of gastrointestinal tract. I am wondering what method tu use, as there is a staining...
17 February 2020 1,979 1 View
Hi, is it possible to activate inflammasome in mice BMDM cells wich are not primed with LPS? We are trying to search for increase of Il1ß after cholesterol crystals incubation but we do not see...
07 February 2020 6,940 4 View
Hello, I was wondering about your opinon on addition of LPS to caerulin in provoking acute pancreatitis (model of repeated caerulin i.p. injection followed by dose of LPS to exacerbate...
04 February 2020 1,432 2 View
Hi! I was wondering how to differentiate between free cholesterol and cholesterol crystals when staining tissues (duodenum). Is use of polarized light mandatory to recognize crystals, or is it...
20 January 2020 5,194 0 View
I would like to stain slides prepared from biopsies of GI tract for cholesterol (and cholesterol crystals), would you recommend any filipin staining protocol ?
16 January 2020 8,613 2 View
