15 Questions 13 Answers 0 Followers
Questions related from Michał Żorniak
Does anyone has some suggestions or experience on stimulation of gallbladder emptying in mouse in vivo? CCK-physiological doses could be an option, as we want to avoid pancreatitis induciton in...
15 April 2020 7,062 0 View
Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??
05 March 2020 5,827 3 View
Hi everyone, I am struggling with visualisation of possible deposition of cholesterol crystals in tissues of gastrointestinal tract. I am wondering what method tu use, as there is a staining...
17 February 2020 2,004 1 View
Hi, is it possible to activate inflammasome in mice BMDM cells wich are not primed with LPS? We are trying to search for increase of Il1ß after cholesterol crystals incubation but we do not see...
07 February 2020 6,958 4 View
Hi, I am working with LDH-Glo (https://www.promega.com/-/media/files/resources/protocols/technical-manuals/500/ldh-glo-cytotoxicity-assay-technical-manual.pdf?la=en) cytotoxicity assay and...
06 February 2020 6,535 1 View
Hello, I was wondering about your opinon on addition of LPS to caerulin in provoking acute pancreatitis (model of repeated caerulin i.p. injection followed by dose of LPS to exacerbate...
04 February 2020 1,445 2 View
Hello there, I wonder has anyone tried to inject substances/drugs into gallblader in mice in vivio? Have anyone encountered protocols or similar studies? Greatly appriciate, Michal
31 January 2020 4,270 0 View
Hi! I was wondering how to differentiate between free cholesterol and cholesterol crystals when staining tissues (duodenum). Is use of polarized light mandatory to recognize crystals, or is it...
20 January 2020 5,206 0 View
I would like to stain slides prepared from biopsies of GI tract for cholesterol (and cholesterol crystals), would you recommend any filipin staining protocol ?
16 January 2020 8,626 2 View
Hi! I am now comparing different effects of LPS stimulation. Should I expect any toxic effect (i.e. seen in FACS analysis) of coincubation of cells other than immune cells (i.e. macrophages)...
17 December 2019 7,576 3 View
Hello everybody, I am currently searching for some oxidative stress markers, which could be measured in frozen serum, after lets say - about 6 month storage in -80 Celsius temperature. That is...
22 May 2016 7,741 4 View
I am interested in starting-up a project investigating propable new pathways of - systemic inflammatory response syndrome (SIRS), - compensatory anti-inflammatory response syndrome (CARS) and -...
26 April 2016 4,194 3 View
I am interested on starting a project considering the topic of GAVE in cirrhotic patients and I am looking for some answers to questions and doubts I have. Does anyone have an opinion on...
04 July 2015 9,525 5 View
Do you have any thoughts on follow-up in patients after liver transplantation in context of fatty liver disease? In light of fact of possible negative influence of immunosupressive drugs (i.e...
15 June 2015 3,683 5 View
Hello, greetings. I was wondering about possible outcomes of estrogen hormones supplementation in light of fatty liver disease in women with PCOS. As PCOS and nonalcoholic fatty liver disease...
27 May 2015 8,754 2 View
