Hi!
I am now comparing different effects of LPS stimulation.
Should I expect any toxic effect (i.e. seen in FACS analysis) of coincubation of cells other than immune cells (i.e. macrophages) with LPS so I can use this group as a positive control for comparison with tested substance? In my case those are human pancreatic ductal cells and I need a postive control for tetsed toxic substance.
Best!
First, your non-immune cells has to have TLR-4, otherwise, LPS doesn't really do a whole lot.
I don't understand what you are looking for. Are you looking for a positive control for cytotoxicity? If so, I won't use LPS. And controls for cytotoxicity depends on how you want the cells to die...
Hello,
I think to get sure whether LPS can affect non-immunological cells directly and indirectly (Through the media). Check if pancreatic ductal cells have LPS receptor in literature then try to culture those cells in media containing LPS. If there is no much difference then I guess you can use it as positive control.
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