How to visualise cholesterol crystals inside tissue/cell?

More Michał Żorniak's questions See All

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

What tools or software can I use in order to visualize EEG brain to brain synchrony?

I want to visualize brain to brain synchrony results (using EEG) as an illustration of two 3D heads with lines connecting the electrodes positions between the two heads where brain to brain...

16 November 2020 7,742 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

Silica gel in the sample on RP-HPLC ?

Hi Guys! Excuse my ignorance but I have little experience with RP-phases. I've managed to initially purify my peptide product using normal phase flash-chromatography. Unfortunately, Ihad to use a...

20 July 2020 7,274 14 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Is there any way to receive Hirsch index excluding self-citations from the Web of Science core collection?

The only thing I see is the Hirsch index value including all citations. Despite looking through the WoS tools I could not find any way to exclude it. However, I'm quite sure there must be some way...

15 June 2020 6,324 2 View

What is the scaling law for thermal damage to biomolecules/proteins from ultrashort heating?

It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...

05 June 2020 6,650 1 View

Challenges in medical education in pandemic era?

These days many of academic teachers around the world have switched to e-teaching due to current epidemiological situation related to SARS-CoV-2. This is a great challenge to both students,...

31 March 2020 2,025 5 View

COVID-19 at psychiatric clinic?

In the current pandemic situation, it is not possible to completely avoid a psychiatric patient carrying COVID-19 or being directly symptomatic. In addition, medical priority is to help anyone....

19 March 2020 4,389 10 View

Cholesterol crystals depletion in tissue of gastrointerstinal tract - microscopic visualisation?

Hi everyone, I am struggling with visualisation of possible deposition of cholesterol crystals in tissues of gastrointestinal tract. I am wondering what method tu use, as there is a staining...

16 February 2020 1,866 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Guenther Juergens

As shown in our publication in

„ARTERIOSCLEROSIS AND THROMBOSIS

Volume: 13 Issue: 11 Pages: 1689-1699

DOI: 10.1161/01.ATV.13.11.1689

Published: NOV 1993“

cholesterol crystals can be easily seen in histological tissue cuts of atheroma as characteristic white needles by means of light microscopy!

Best Regards,

Günther

Michał Żorniak

Thank you so much Guenther!

I will try this and let know if it works for me!

Best Guenther Juergens

Vitaly Borovik

V. S. Borovik et al., Visualization during Crystallization in Minkowski Spacetime V.S. Borovik, V.V. Borovik and D.A. Skorobogatchenko Solid State Phenomena in the year 2017 Vol.269. PP7-14. DOI: 10.4028/www.scientific.net/SSP.269.7(Scopus)