The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

What tools or software can I use in order to visualize EEG brain to brain synchrony?

I want to visualize brain to brain synchrony results (using EEG) as an illustration of two 3D heads with lines connecting the electrodes positions between the two heads where brain to brain...

16 November 2020 7,742 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

Silica gel in the sample on RP-HPLC ?

Hi Guys! Excuse my ignorance but I have little experience with RP-phases. I've managed to initially purify my peptide product using normal phase flash-chromatography. Unfortunately, Ihad to use a...

20 July 2020 7,274 14 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Is there any way to receive Hirsch index excluding self-citations from the Web of Science core collection?

The only thing I see is the Hirsch index value including all citations. Despite looking through the WoS tools I could not find any way to exclude it. However, I'm quite sure there must be some way...

15 June 2020 6,324 2 View

What is the scaling law for thermal damage to biomolecules/proteins from ultrashort heating?

It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...

05 June 2020 6,650 1 View

Challenges in medical education in pandemic era?

These days many of academic teachers around the world have switched to e-teaching due to current epidemiological situation related to SARS-CoV-2. This is a great challenge to both students,...

31 March 2020 2,025 5 View

COVID-19 at psychiatric clinic?

In the current pandemic situation, it is not possible to completely avoid a psychiatric patient carrying COVID-19 or being directly symptomatic. In addition, medical priority is to help anyone....

19 March 2020 4,389 10 View

How to visualise cholesterol crystals inside tissue/cell?

Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??

04 March 2020 5,669 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View