Hi everyone,

I am struggling with visualisation of possible deposition of cholesterol crystals in tissues of gastrointestinal tract.

I am wondering what method tu use, as there is a staining with filipin but from what I undestood - this will stain ALL OF FREE cholesterol (so also this deposited in cells), and I am not sure if that will make crystals vivible.

Second option - is using simply polarized light microscope, and here I would like to ask if anyone has experience or protocol he could share?

best!