23 Questions 33 Answers 0 Followers
Questions related from Meriem Fassatoui
Hello, In order to delimit the chromosomal region of TLR4 gene that it will be investigated from chip data ,is it correct to remove 50 kb upstream and i add 50 KB downstream : example...
18 November 2018 5,339 0 View
Hello , I'm asking you why they consider that Wilcoxon test and U Mann Whitney test are the same ? Can we use this test to examine 2 numeric variables for independent groups ? Thank you
11 September 2018 6,312 3 View
hello, i want to know why can't we talk about exon in bacteria ? It was reported in the literature that this term: exon is specific to eucaryotic organisms,. but we can find introns in some...
02 June 2017 3,426 8 View
Hello, i want to know what are those 3 concepts associated to coverage namely: Fold Coverage (number + X ) -‐ Breadth of Coverage -‐ Depth of Coverage how can we calculate the...
09 May 2017 7,591 2 View
Hello, I want to sequence rRNA16S of bacteria by means of next generation sequencing technic using Illumina MiSeq technology. In articles i see that they choose hypervariable region V4 or V6...
22 December 2016 5,038 1 View
Good afternoon, I inserted all SNPs in haploreg v4.1 tool, i obtained colors and codes, i want to know what does every code refer to. Thanks
06 October 2016 1,767 1 View
Hello, I'm working on the gene TLR4 in human, in order to delimit the chromosomal region i removed 50 kb upstream and i added 50 KB downstream : example 117,704,372-117,716,871 after edit:...
03 September 2016 3,683 0 View
Hello, Can you give me the name of a Data base created to estimate the incidence of type 2 diabetes like GLOBOCAN do for cancer ?
18 August 2016 5,216 3 View
Hello, Can you tell me please how can i identify in which direction the sequence of DNA will be inserted into the vector (for example pBluescript) Please look at the attached file below...
22 July 2016 9,541 8 View
Hello, i wish to compare the allelic frequencies of SNPs extracted from HAPMAP PROJECT between populations, i take always into consideration the GMAF extracted from dbSNP, but sometimes i find for...
22 June 2016 7,837 0 View
Hello, I conserved the extracted microbial DNA from kit in -20°C (in TE BUFFER), after that i was obliged to transfer the microbial DNA to + 4°C because i can't ensure the transport from a place...
25 April 2016 7,466 3 View
Hello, In order to compare the distribution of the Minor allele frequency of SNPs detected in a gene X between two populations for example For the rs X MAF % EUROPEAN 1.786/ EAS : 0/ The...
01 February 2016 2,721 2 View
Hello, Can anyone give me the link of a tool able to detect SnpS of exome data for population for exemple for TLR4? American...N..SNPs African N Snps European N SNPs Asiatic N...
18 January 2016 1,455 3 View
hello, I created .ped and .info files, now i want to know how can i create a haploview map using gplink ? i need to know which order can i integrate in the server to obtain results? I need more...
27 August 2015 3,491 5 View
Hello, I want to ask you about the GC% of human genome, origin and susceptibility to be involved in diseases (Diabetes..) in case of lopsided % Thank you
14 August 2015 4,299 1 View
Hello I have to analyse exomes of different genes il6, CD14, HLA-II... (plink tutorial)..I wonder if it is possible to obtain reliable genotyping data from data exomes ? Thanks
21 July 2015 9,514 2 View
Hello, I want to know which formula can i applicate to calculate "the power analysis " of a scientific project, how many patients should be recruted (cohorts) in order to detect linkage with a...
20 June 2015 2,044 7 View
hello, I need primers (f+r) and probes specific to firmicutes and the Strain Akkermansia muciniphila in order to work through QPCR (TAQMAN method) can you provide me articles in which the authors...
14 April 2015 8,019 2 View
Hello, In order to conduct QPCR (TAQMAN method) manipulations I will need bioinformatics tools to check the specificity of the probes and primers for selected strains. what these letters refer to...
12 April 2015 9,093 6 View
I wish to sequence the bacterial DNA amplification products amplified by real-time quantitative pcr (SYBR Green method) for that I need a kit that would allow me to purify the amplified...
08 February 2015 4,870 2 View
I extracted DNA from bacteria by classical approach or protocol phenol/chloroform method. Is it possible that this DNA is inactivated after extraction? If yes, which is the component that has...
18 July 2014 1,869 14 View
I plan to clone a bacterial gene. I ordered the plasmid vector pGEX but I just learned that the order will arrive in six months. Who can offer me a protocol so that I can build a cloning vector...
19 April 2014 5,532 8 View
HELLO, I would like to know what are the statistical tests used to calculate the power of a study. EG : i would like to calculate the minimum number of samples that should be collected to...
01 January 1970 1,283 7 View
