Hello,
In order to conduct QPCR (TAQMAN method) manipulations I will need bioinformatics tools to check the specificity of the probes and primers for selected strains.
what these letters refer to ? (6-FAM), (BHQ-1)
Probe bacteroidetes
(6-FAM)-CCATTGACCAATATTCCTCACTGCTGCCT-(BHQ-1)
thanks