Who can advise me a kit that would allow to purify amplicons from quantitative real-time pcr (SYBR Green method)?

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Could anyone provide me a spreadsheet to present the composition of sulphides in diagrams?

10 April 2024 6,290 0 View

How to calculate the Mean normal partial thromboplastin time ?

Is it like MNPT (geometrical mean of at least 20 healthy subjects) ? or it's the arythmetical mean ? can a pool of healthy subjects' plasma be considered as the reference APTT for calculating APTT...

01 April 2024 1,846 0 View

What are teachers' motivation strategies?

This research aims to investigate the relationship between teachers' intrinsic motivation strategies and EFL students' outcomes in Algerian university

22 March 2024 3,670 0 View

How can idownload MAUD software in my laptop?

how can i download MAUD software in my pc?

03 October 2023 8,919 3 View

Is there any Online database of phenolic compounds? (Except phenol-explorer) ?

I am searching for an online database of phenolic compounds extracted from plants which contains their UV spectra (or at least their λmax). In the database phenol-explorer, there is almost...

24 April 2023 3,774 3 View

Why the hydrophobic interactions are not linked to the interert residue in the Yasara software ?

Hello everyone, I have a question about the YASARA software. I want to discover the hydrophobic interactions between some protein residues. These interactions are not linked to the interest...

14 February 2023 4,936 1 View

Is there any relationship between Virilio's dromology approach and Hartmut's acceleration paradigm ?

I think loudly about the two concepts of time I.e : "dromology and acceleration".. through an interdisciplinary framework between philosophy and politics..

07 October 2022 6,194 0 View

Cooja - How to assign different services to a mote/node?

I need to create a network of 14 motes.each mote is either a service provider or service requester. A node provide a service or services.I want each mote to provide 3 services withS1 = 0.90, S2 =...

14 August 2022 7,486 1 View

Why autodock vina does not generate the dlg files for acids such as coumaric, caffeic, gallic ...etc?

Why autodock vina does not generate the dlg files foor acids such as coumaric, caffeic, gallic ...etc?

02 August 2022 5,239 2 View

PCR products from Yeast DNA (ITS) are not intense?

Dear Community, I extracted DNA from yeasts and performed a PCR with ITS primers. The PCR bands are not intense and do not allow sequencing. I've tried to extract DNA with a lithium protocol, but...

11 June 2022 503 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View