Hello,

In order to delimit the chromosomal region of TLR4 gene that it will be investigated from chip data ,is it correct to remove 50 kb upstream and i add 50 KB downstream :

example 117,704,372-117,716,871

after edit: 117,654,372-117,766,871

How can i predict if the substraction and addition of 50 KB is good or not suitable ? Can you communicate to me a reliable tool which allows me to do this task?

If i have to investigate the same gene from exome data is it okay if i remove 2kb to get upstream of the transcription sites of genes and add 2kb ?

Thank you