Hello,

I'm working on the gene TLR4 in human, in order to delimit the chromosomal region i removed 50 kb upstream and i added 50 KB downstream :

example 117,704,372-117,716,871

after edit: 117,654,372-117,766,871

Do you have an idea about a tool in which i can insert the editing chromosomal region in order to look for the presence of pseudogenes which can interfere with TLR4 ?

How can i predict if the assessment of removal and addition of 50 KB is good or not suitable for this work ? Can you communicate to me a reliable tool which allows me to do this task? I saw in articles for example that they remove 2kb and add 2kb .

Thank you