Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

What are the causes of hyphae granulation?

Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...

05 February 2021 5,523 4 View

How to calculate the field capacity??

28 December 2020 1,521 2 View

How to decrease the difference between the simulation result obtained by comsol and analytical result ?

Hello, I am working on a piezoelectric energy harvester in comsol so I coupled the electrical circuit interface with the piezoelectric device multiphysics. I tried to confirm these results with...

07 December 2020 1,007 2 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

Hormone levels in male mice ?

Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.

20 September 2020 1,035 3 View

How can I fix the connvergence error!?

opt freq gaussian calculation is performed, I got normal termination , however, the structure has nottotaly converged. because I had no convergence in Maximum Displacement! how can I solve this...

06 September 2020 4,283 3 View

Determination of activated carbon surface functional groups by Boehm titration ?

i did titration to determine functional groups on the surface of activated carbon, when the titration was done on Na2HC03 and NaOH by HCl, a big volume was necessary to reach equilibrium, and...

13 July 2020 1,022 2 View

Keras code python ?

Hello, I am using a VGG16 on keras to do some image classification on a dataset, What does it mean that the validation loss is smaller all the time than the train loss?. Is 80% a reasonable...

11 July 2020 569 6 View

How to get PWM signals from Dspace?

I'm working on dSPACE 1104 with DSP-4 channel PWM generation bloc. I need to use the PWM signals in other programs but i didn't know how to get them since this bloc has no outputs on matlab...

04 March 2020 2,854 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View