i want to know what are those 3 concepts associated to coverage namely: Fold Coverage (number + X ) -‐ Breadth of Coverage -‐ Depth of Coverage how can we calculate the coverage of a gene and what are the utilities of this task ?
The term "coverage" is taken in its literal meaning i.e. how much of the intended genome is covered in your sequencing. Though coverage can be expressed in terms of "breadth" or "depth", it is taken in terms of depth by default. The depth of coverage indicates how many times a nucleotide in the intended genome has got sequenced. The breadth of coverage is portion of the intended genome covered at some depth in a dataset.