Mehjabeen Javed

16 Questions 28 Answers 0 Followers

Questions related from Mehjabeen Javed

Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.

10 October 2015 6,269 3 View

Can anybody tell me how is the tolerable weekly intake calculated for metals?

I am working on toxicology of trace elements.

01 January 2014 8,176 3 View

What statistical test is suitable for comparing three polluted water sites?

I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...

01 January 2014 4,099 11 View

I am working on protein of fish liver and muscles serum after intoxication by a pollutant. What do these parameters tell about health of fish?

I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...

01 January 2014 2,894 6 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

12 December 2013 2,903 14 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...

11 November 2013 3,614 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

11 November 2013 5,964 5 View

Can someone advise on Students' t test (2 tailed) in SPSS (version 16)?

I attached the results some one please tell me that which value i have to see in the result to know whether it is significant or not. Is it that which i marked in red?

10 October 2013 3,496 7 View

Can someone advise on the choice of buffer, which will work for both glycogen and enzyme GOT, GPT and Alkaline phosphatase estimation?

I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...

10 October 2013 1,362 2 View

Is it feasible to determine levels of GPT, GOT and total protein from the dried fish tissues?

I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?

09 September 2013 9,498 7 View

I did rotary microtomy on gill section of fish channa punctatus but I'm not getting what deformities are present in it.

I did microtomy by paraffin embedding and the section I get is observed under Nikon eclipse 80i microscope at 40x. I attached the figure in the hope that someone could tell me if the deformities...

08 August 2013 4,186 4 View

What is the significance of bioconcentration factor, bioaccumulation factor and metal pollution index?

What will I deduce from them?

06 June 2013 5,076 7 View

How can the quantification of amino acids be performed?

I want to estimate the amino acids from fish muscle both qualitatively and quantitatively. Qualitative analysis can be done by HPLC but I don't know how I could work out the quantity? Can anyone...

06 June 2013 8,537 7 View

Could anyone provide me with the protocol for sample preparation (fish muscle) amino acid estimations by HPLC/ ion exchange chromatography?

I want to estimate amino acids from fish muscle therefore need to know the protocol of sample preparation for both qualitative and quantitative estimations either by HPLC / ion exchange...

05 May 2013 2,823 0 View

Could anybody tell me that what the wavelength, flow rate, column, solvents A&B etc. will be for the analysis of amino acids from fish muscle by HPLC?

I am working on fish health and have to analyse the amino acids profile of fish both qualitatively and quantitatively. So would anybody help me that what the HPLC working conditions will be for...

05 May 2013 7,723 6 View

Contamination factor/pollution load index, are these indexes valid only for sediments?

I am working on aquatic toxicology and want to calculate these indexes for water samples. Do they work for water?

04 April 2013 9,511 1 View