Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.

09 October 2015 6,227 3 View

I am working on protein of fish liver and muscles serum after intoxication by a pollutant. What do these parameters tell about health of fish?

I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...

31 December 2013 2,853 6 View

What statistical test is suitable for comparing three polluted water sites?

I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...

31 December 2013 4,062 11 View

Can anybody tell me how is the tolerable weekly intake calculated for metals?

I am working on toxicology of trace elements.

31 December 2013 8,138 3 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

11 December 2013 2,853 14 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...

10 November 2013 3,571 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

10 November 2013 5,923 5 View

Can someone advise on the choice of buffer, which will work for both glycogen and enzyme GOT, GPT and Alkaline phosphatase estimation?

I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...

09 October 2013 1,322 2 View

Can someone advise on Students' t test (2 tailed) in SPSS (version 16)?

I attached the results some one please tell me that which value i have to see in the result to know whether it is significant or not. Is it that which i marked in red?

09 October 2013 3,453 7 View

Is it feasible to determine levels of GPT, GOT and total protein from the dried fish tissues?

I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?

08 September 2013 9,460 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View