I am working on toxicology of trace elements.
[A]The daily intake rate of metals (DIR) was calculated by
the following equation:
DIR =C(metal) .D(food intake)/ B(average weight)
Where C(metal) is Heavy metal concentration in plants
(μg/g).D(food intake) is daily intake of vegetable (kg/person)
B(average weight) is average body weight.
{B}The average adult body weights were considered to be
55.9kg, while average daily vegetable intakes for adults is
considered to be 0.345 kg/person/day, respectively
[C]The true intake using the arithmetic mean according
to Parkhurst (1998) was calculated by multiplying
contaminant level i.e. (yearly intake of rice, cassava and
fruits multiplied by heavy metal contaminant for each of
the food items).
[C] In all the estimated or calculated levels
of lead , cadmium and nickel in the food samples yearly averages
as quoted by various workers were used .
[D] Two types of foods are commonly consumed :
(i) RICE& ORANGES(Primarily Indian diet
(Ii) Manihot spp&Bannana( For other countries)
[E]
(I) First type of diet:
Amount of Pb, Cd and Ni in Rice( mg/Kg)=61.17, 0.0 and 0.0
Pb,Cd and ,Ni In Citrusa(mg/g)= 0.69,0.24 and 0.0
(II)Second type of diet
Amount of Pb, Cd and ,Ni in Manihot spp ( mg/Kg)=0.33,0.10 and 0.24
Amount of Pb, Cd and ,Ni in Bannana ( mg/Kg)=0.46,0.0 and 0.0
[F]
(I) First Type of diet
Total Rice consumption/year=24.8Kg
Suppose Citrus fruit cosumed daily=200g
(II)Again in Second Type of diet
Total Manihot spp consumption/year=214Kg
Suppose Bannana frit cosumed daily=2oog
[H] Daily in take of the metals
(I)First Type of diet
[Pb]==24:8kg/365days x 61 .17 mg/kg
+ 200gx0:69mg/kg =3:53g/kg
[Cd]= 24:8kg/365days x0:0mg/kg+ 200gx0.24mg/kg =0.034g/Kg.
[Ni]= 24:8kg/365days x0:0mg/kg+ 200gx0.0mg/kg =0.0g/Kg.
(II)Second Type of diet
[ Pb ]= 214kg/365days x0:33mg/kg
+200gx0:46mg/kg= 19:42g/kg
[ Cd ]= 214kg/365days x0:10mg/kg
+200x0= o.049g/Kg
[ Ni ]= 214kg/365days x0:30mg/kg
+200 x0 = 0.18g/Kg
Note: These are the values of intake of the metals / day. Multiply by 7 to obtain per week.
Pls find reference to authenticate the answer
Research article
Heavy metals health risk assessment for population via consumption of food crops and fruits in Owerri, South Eastern, Nigeria
Orish Ebere Orisakwe1*, John Kanayochukwu Nduka2, Cecilia Nwadiuto Amadi1,Daniel Onyekachi Dike3 and Onyinyechi Bede3
You have to know first the allowable daily intake of each metal, so calculate from the average weight of a human and a ration of that food
I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.
09 October 2015 6,227 3 View
I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...
31 December 2013 2,853 6 View
I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...
31 December 2013 4,062 11 View
I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...
11 December 2013 2,853 14 View
I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...
10 November 2013 3,571 2 View
I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?
10 November 2013 5,923 5 View
I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...
09 October 2013 1,322 2 View
I attached the results some one please tell me that which value i have to see in the result to know whether it is significant or not. Is it that which i marked in red?
09 October 2013 3,453 7 View
I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?
08 September 2013 9,460 7 View
I did microtomy by paraffin embedding and the section I get is observed under Nikon eclipse 80i microscope at 40x. I attached the figure in the hope that someone could tell me if the deformities...
07 August 2013 4,143 4 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...
01 March 2021 1,899 2 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...
27 February 2021 4,285 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View
I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....
24 February 2021 4,428 3 View