Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.

09 October 2015 6,227 3 View

I am working on protein of fish liver and muscles serum after intoxication by a pollutant. What do these parameters tell about health of fish?

I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...

31 December 2013 2,853 6 View

What statistical test is suitable for comparing three polluted water sites?

I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...

31 December 2013 4,062 11 View

Can anybody tell me how is the tolerable weekly intake calculated for metals?

I am working on toxicology of trace elements.

31 December 2013 8,138 3 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

11 December 2013 2,853 14 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...

10 November 2013 3,571 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

10 November 2013 5,923 5 View

Can someone advise on the choice of buffer, which will work for both glycogen and enzyme GOT, GPT and Alkaline phosphatase estimation?

I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...

09 October 2013 1,322 2 View

Can someone advise on Students' t test (2 tailed) in SPSS (version 16)?

I attached the results some one please tell me that which value i have to see in the result to know whether it is significant or not. Is it that which i marked in red?

09 October 2013 3,453 7 View

I did rotary microtomy on gill section of fish channa punctatus but I'm not getting what deformities are present in it.

I did microtomy by paraffin embedding and the section I get is observed under Nikon eclipse 80i microscope at 40x. I attached the figure in the hope that someone could tell me if the deformities...

07 August 2013 4,143 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View