Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.

09 October 2015 6,227 3 View

I am working on protein of fish liver and muscles serum after intoxication by a pollutant. What do these parameters tell about health of fish?

I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...

31 December 2013 2,853 6 View

What statistical test is suitable for comparing three polluted water sites?

I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...

31 December 2013 4,062 11 View

Can anybody tell me how is the tolerable weekly intake calculated for metals?

I am working on toxicology of trace elements.

31 December 2013 8,138 3 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

11 December 2013 2,853 14 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...

10 November 2013 3,571 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

10 November 2013 5,923 5 View

Can someone advise on the choice of buffer, which will work for both glycogen and enzyme GOT, GPT and Alkaline phosphatase estimation?

I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...

09 October 2013 1,322 2 View

Can someone advise on Students' t test (2 tailed) in SPSS (version 16)?

I attached the results some one please tell me that which value i have to see in the result to know whether it is significant or not. Is it that which i marked in red?

09 October 2013 3,453 7 View

Is it feasible to determine levels of GPT, GOT and total protein from the dried fish tissues?

I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?

08 September 2013 9,460 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View