I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like 0.211 in serum and 1.4 in liver. What does this mean? Or what is the significance of A/G ratio? Could anybody please elaborate.
Consult Hoseini, S M andR Tarkhani (2013) Effect of short term...........Carassius auratus. Aquaculture Res. 44 (6) 869-875.
Hello,
I'm here :).
As I worked in some fish species, I know the A:G ratio in fish is not similar to human! Albeit, I used human diagnostic kits for assay of total protein and albumin. Maybe these kits are not reliable for fish (although I believe human total protein and albumin kits are reliable for fish, based on my experiences). However, you can check the kits validity before use.
Now, we assume your assay is valid. The interpretation is not easy. Total protein in fish serum is affected by many factors are not considered by some researchers. First, feed ration and fasting! it greatly affect serum total protein and globulin. Albumin is stable, almost. However, since you do not determine protein fractions, you can't conclude if total protein increase or decrease is a negative or positive indicator.
Albumin is hardly change. So, the change in albumin, especially decrement is a sign of problem in fish. Albumin fall occurs mainly due to liver damage or hypoosmotic shock.
Just let me know what you mean by "liver A:G ratio"? How did you measured that?
In the homogenate of fish liver albumin and globulin were estimated and so their ratio. I would like to read some of your papers related to protein fractions in fish. Could you please send to me via mail. They are not open access.
Thank you
Hi, yes. However, I can't send you any massage in researchgate. Please follow me to enable me sending.
My email is [email protected] , you can contact and follow me. Thanks. V K DAS
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