I am working on fish health and have to analyse the amino acids profile of fish both qualitatively and quantitatively. So would anybody help me that what the HPLC working conditions will be for analysis of amino acids in fish muscle?
1st You need to optimize ur mobile phase. try different solvent systems and then select the best system and proceed for other parameters. Prefer phosphate buffers. Gud luk
Thanks to all. I am not doing it by myself but do it through paid service in CDRI, Lucknow. For service a form has to be filled which needs all these specifications.
could any one of you provide me the protocol for sample preparation (fish muscle) for both qualitative and quantitative estimations by HPLC/ ION EXCHANGE CHROMATOGRAPHY.