Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

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I am working on protein of fish liver and muscles serum after intoxication by a pollutant. What do these parameters tell about health of fish?

I worked out the total protein, albumin, globulin and albumin:globulin ratio. I read that for humans the ideal albumin:globulin ratio is 2:1. In my study i observed very very small value like...

31 December 2013 2,853 6 View

What statistical test is suitable for comparing three polluted water sites?

I worked on 3 water bodies which are polluted by different sources and collect channa punctatus from each water body to check its health. I collected water and checked 7 heavy metals in these...

31 December 2013 4,062 11 View

Can anybody tell me how is the tolerable weekly intake calculated for metals?

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31 December 2013 8,138 3 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

11 December 2013 2,853 14 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

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10 November 2013 3,571 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

10 November 2013 5,923 5 View

Can someone advise on the choice of buffer, which will work for both glycogen and enzyme GOT, GPT and Alkaline phosphatase estimation?

I want to estimate the glycogen by anthrone reagent using TCA for making homogenate then centrifuge and the enzymes (GOT, GPT, ALP) from fish liver and muscle and kidney. Is TCA suitable for...

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Is it feasible to determine levels of GPT, GOT and total protein from the dried fish tissues?

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Can I use TCA instead of TFA for protein analyis in RP-HPLC?

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Chithan C Kandaswami

How are you searching? You may want to use PUBMED and AGRICOLA. You would find assays for different digestive enzymes in the series, "Methods in Enzymology", a long-standing series. http://www.ncbi.nlm.nih.gov/pubmed

THE CONCENTRATIONS OF PROTEASES, AMYLASE, AND LIPASE IN CERTAIN MARINE FISHES. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.314.6521&rep=rep1&type=pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762538/ Digestive enzyme activities and gastrointestinal fermentation in wood-eating catfishes

http://www.ncbi.nlm.nih.gov/portal/utils/pageresolver.fcgi?recordid=5619ee7835fd1e11e9c52d5a Effects of Replacement of Fish Meal by Soy Protein Isolate on the Growth, Digestive Enzyme Activity and Serum Biochemical Parameters for Juvenile Amur Sturgeon (Acipenser schrenckii).

Ashok Kumar Nadda

For spectrophotometric analysis various popular mehtods are available

Lipase: Winkler and stuckmann 1979

Proteases Casien hydrolysis assay

Good luck