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Questions related from Kyungsun Ha
Greetings. In formaldehyde test, there are control solution for 2.5ppm, 5ppm, 10ppm... and so on. But formaline contains maximum 37~38% of gas formaldehyde, how can I make n(number) ppm...
18 April 2019 7,802 4 View
Greetings. I founded protease K solution, but have some qusetion... 1. what's different between Protease K solution and Protease K buffer? (some solution enclude CaCl2 and no EDTA, or enclude...
15 January 2019 775 2 View
Greetings. I preparated genomic DNA with Pig stool, but the concentration got lower. about 3~4 (ng/ul) when I measured by nanodrop, A260 / A280 and A260 / A230 did not come out well. Especially...
09 January 2019 2,616 5 View
Greetings. this is PEG(polyethylene glycol) particle with COOH, that photo is when I run experiment, conjugate NH2-modified oligonucleotides and hybridized with FAM-DNA. but DNA was spotted....
28 September 2017 7,243 0 View
Greetings! Here is tuesday now.. After coujugation of amine coupled oligonucleotide to PEG particle, we hybridized and it seemed like fluorescence this point, possibly DNA also overall negative...
19 September 2017 8,774 3 View
Amino modified oligonucleotide and PEG gel (- COOH) were conjugated using NHS / EDC, but the gel was not uniformly fluorescent but partial partial points could be taken. It seemed like I took a...
13 September 2017 7,128 0 View
I will conjugate with COOH-particle(hydrogel) woth streptavidin using NHs/EDC, but it will conjugated? streptavidin is no modified, I use streptavidin in sigma aldrich(85878-1mg).
11 September 2017 2,046 1 View
Before ask, My scheme is 'COOH particle-NH2 oligonucleotide conjugation'->'DNA:DNA hybridization' In hybridization, use saline sodium citrate buffer because It provides ion(salt) for stronger...
28 August 2017 3,864 0 View
In NHS/EDC protocol, there are two buffers. coupling buffer and activation buffer. (+washing buffer) And, I will conjugate PEG-COOH to amine-modified oligonucleotide. In the NHS / EDC reaction...
09 August 2017 6,012 2 View
I will conjugate PEG gel with amine modified oligonucleotide. (particle in paper. they used PEG200 and PEG700DA) I heard about EDC/NHS conjugates amine and carboxylic acid, so carboxylation of PEG...
08 August 2017 9,175 2 View
Once I know how the NHS / EDC works. However, the subject I am going to experiment with is the amino-modified oligonucleotide (C6, 5 ') ... the protocol is related to the protein ... (I have not...
07 August 2017 3,569 3 View
Greetings. I saw at linked article, human gene 1 copy=3.3pg. but, I don't know concentration of 3.3pg single molecule DNA. so what's concentration of it? (in molarity) Actually, I know, RNA...
18 July 2017 9,051 10 View
I did surface plasmon resonsnce, but I have a problem... Progress is PBS-Streptavidin-PBS-Linker-1X TBS-DNA. But this is not very important. Because all graphs, whether buffers or samples, look...
19 June 2017 7,501 1 View
at Qiagen therascreen pyro kit, there a dNTPs. but in there, dATP is modified, dATP alpha S. So I found why uses dATP alpha S, but there's no answer... why uses dATP alpha S in pyrosequensing?
13 June 2017 8,098 6 View
Greetings, here is friday morning! Nothing but, there is a question as to the microarray. I use Arrayit micro spotting solution 2X plus. As you can see in the photograph, after baking recommended...
31 March 2017 6,826 7 View
I just changed target DNA solution from DMSO to arrayit micro spotting solution 2X plus, and after washing(N2 gas dry) I stacked my slides, but it spreaded so I cannot see dots.. spotting is Okay...
29 March 2017 5,329 2 View
Greetings! I want to know about glass petri dish.. I used plastic petri dish at baking step of microarray, but it was distorted... so I want to use glass dish. But, is it Okay I reuse that dishes?...
22 March 2017 5,526 1 View
I heard about, scanned with microarrayed slide like show image below, black background+fluorescence dots. But what I saw is all-of-white slide with black frosted ends(conatantly black, below). I...
17 March 2017 593 6 View
Greetings! Today I come to ask something.. I will run experiment PI staning for microarrayed slide, because I cannot see fluorescence(cy3). but there are protocol only for animal cell... my DNA...
14 March 2017 6,248 2 View
I solute both target(which adhered with glass) and probe in 50% DMSO. But, when I spotting DNA and baking in 80 degree, it spreaded and mixed. DMSO has low surface tension? where you solute your...
14 March 2017 2,107 0 View
Greetings! It's Monday.. Actually, I ruined my first microarray because 'poor signal'... I find where I printed DNA very very difficultly, but it wasn't shining. label is cy3, concentration is...
13 March 2017 1,772 2 View
I want to buy hybridization chamber for maintain humidity, but it was already discontinued. I don't want to find slide with chamber. then, what is hybridization bottle? When I can use It? I can...
06 March 2017 1,860 0 View
Greetings. Here is Korea. I ordered from the sigma molecular beacon, Singapore can not be synthesized by the molecular beacon estimates put the headquarters in the United States. In the middle of...
06 March 2017 6,895 0 View
http://compbio.dfci.harvard.edu/compbio/research/labprotocols/contentBlocks/0/contentBlock_files/file/MicroarrayHybridization.pdf I found this at google, and I will use this protocol in...
27 February 2017 8,804 0 View
I have a question about protein structure prediction, during reading paper. Enzymes need co-factors for activate, and it have a active site and co-factors binding area. So, how can teach for AI...
01 January 1970 2,838 1 View
Greetings. I am search and read about papers about orphan receptor now. And some question floated in my head, that is, 'why orphan receptor's ligand not yet discovered?'. I've heard that due to...
01 January 1970 7,173 3 View
