Greetings.
I preparated genomic DNA with Pig stool, but the concentration got lower. about 3~4 (ng/ul) when I measured by nanodrop, A260 / A280 and A260 / A230 did not come out well. Especially A260 / A230 was 0.4~0.5. when you prep at stool, A260.A230 was too low under 1.0? During experiment, I filtered DNA two times.
You have lot of contamination. The normal value for 260/230 should be around 2 for good nucleic acid extraction.
stool have many things but... it is so low concentration and too many contaminant.. then, during prep, can contaminant decrease filtering more than 2 times?
stool+1X PBS 1ml->vortexing
after vortexing, centrifuge ans cell lysis, put elution buffer and spin column uses first time, washing two times and elute(use a spin column).
It is tricky to answer since there were numerous things unclear. I suggest you to stricter in lesser contamination especially after lysis process.
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