I solute both target(which adhered with glass) and probe in 50% DMSO. But, when I spotting DNA and baking in 80 degree, it spreaded and mixed. DMSO has low surface tension? where you solute your DNA for microarray?
If the depolarization ratio of a Raman peak is less than 0.75, it is a polarized band, and if it is greater than 0.75, it is a depolarized band.
04 May 2024 2,829 0 View
Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...
27 April 2024 4,710 1 View
Good evening (at least in me time-zone) I have been having some trouble with some routine Immunological staining. What the ideal conditions for IHC slides before staining is? Should I leave these...
17 April 2024 6,831 2 View
Hi. I'm a new researcher trying to get ready to publish my first paper in the SCI Journal. Since my research is a combination of using psychological methods to screen obese and normal people...
18 March 2024 3,434 2 View
Hi, I'm trying to figure out the lowest off-current level using the RPI poly-Si TFT model (Level 62). From what I've done, changing the parameters in the model doesn't make the off-current figure...
22 January 2024 1,300 0 View
Here are 3 models to emulate
30 December 2023 6,550 3 View
The mice were induced to have type 2 diabetes by feeding high-fat food and injecting low doses of STZ.
07 December 2023 5,690 1 View
Inhibition of alpha-glucosidase Enzyme in invitro I read a few articles and saw that the enzyme concentration they usually use is 0.1U or 0.2U but some articles use 1U. So which one is better and...
01 December 2023 4,905 1 View
What macro factors affect to future coffee price (ICE Future)?
15 November 2023 7,833 1 View
I am using lectin-based ELISAs to probe the glycans of different glycoproteins. I had successful data that showed clear differences in the lectin binding profiles/patterns. However, I wanted to...
08 November 2023 1,632 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View