If PI(propidium iodide) bind on dsDNA?

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Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

How to avoid protein precipitation during Ni-NTA binding step?

Hi everyone, I am purifying an 18 kDa protein (his-tag). The protein expresses very well and I am able to get a clean 18kDa band in SDS Page gel after purification (attached picture). However,...

11 October 2020 3,189 6 View

Whether analysis with CB-SEM by AMOS will have better results comparing with PLS-SEM?

With your experiences, which one will have better results, CB-SEM or PLS SEM?

15 September 2020 8,092 5 View

Is there In-plane anisotropy of Amorphous CoFeB thin film?

I made CoFeB thin film on PMMA. The film thickness is from 100 nm to 10 nm. To measure magnetic properties, an M-H loop according to the angle was obtained using a VSM. However, the graph by angle...

27 July 2020 1,480 3 View

How to determine intention to use a new technology?

Hello, I am doing a research on new technologies and I would like to know how to determine the percentage of people that intend to use a new technology. I know about models like TAM, UTAUT, UTAUT2...

26 February 2020 1,381 10 View

I'm looking for an antibody. can I know where to buy?

Hello, I'm looking for some antibody, CLB-CAg 69. it is a anti-factor VIII antibody whose epitope is A2 domain of FVIII. However, I cannot get this. Can I know where to buy?

15 January 2020 3,325 3 View

"Safe" value of Vgs in sub-threshold design (4.5nUT < Vgs - Vth < -2nUT)?

Hello, Usually, by biasing Vgs < Vthreshold of MOSFET, we can push MOS into sub-threshold region. My curious question is: How much value of Vgs is "good" as a "rule of thumb"? I have referenced...

29 December 2019 9,143 1 View

Can an associate editor become in charge of a submitted manuscript where 1/4 of the total references were associated with his/her own??

Is it appropriate to have Person A as an Associate Editor of a submitted manuscript - where 1/4 of the total references are his/her own and who has been a co-author with the corresponding author...

14 December 2019 7,092 3 View

Which paper/book is best to study sub-threshold operation and model of MOSs devices?

Dear all, There are lots of published work on sub-threshold operation and models of MOS devices (example: on Journal of Solid-State Circuits, IEEE). For a good and highly-credited one, could you...

08 December 2019 5,968 5 View

What is your recommendation for an English editing service?

As I am not a native English speaker, normally, I am advised to use English editing services before submitting my manuscript to the journal. Recently, I got some bad experiences with the service I...

28 November 2019 7,039 11 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View