I will conjugate with COOH-particle(hydrogel) woth streptavidin using NHs/EDC, but it will conjugated? streptavidin is no modified, I use streptavidin in sigma aldrich(85878-1mg).
Some will conjugate but i don't expect a good yeld.
Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...
06 January 2021 7,247 3 View
Hi everyone, I am purifying an 18 kDa protein (his-tag). The protein expresses very well and I am able to get a clean 18kDa band in SDS Page gel after purification (attached picture). However,...
11 October 2020 3,189 6 View
With your experiences, which one will have better results, CB-SEM or PLS SEM?
15 September 2020 8,092 5 View
I made CoFeB thin film on PMMA. The film thickness is from 100 nm to 10 nm. To measure magnetic properties, an M-H loop according to the angle was obtained using a VSM. However, the graph by angle...
27 July 2020 1,480 3 View
Hello, I am doing a research on new technologies and I would like to know how to determine the percentage of people that intend to use a new technology. I know about models like TAM, UTAUT, UTAUT2...
26 February 2020 1,381 10 View
Hello, I'm looking for some antibody, CLB-CAg 69. it is a anti-factor VIII antibody whose epitope is A2 domain of FVIII. However, I cannot get this. Can I know where to buy?
15 January 2020 3,325 3 View
Hello, Usually, by biasing Vgs < Vthreshold of MOSFET, we can push MOS into sub-threshold region. My curious question is: How much value of Vgs is "good" as a "rule of thumb"? I have referenced...
29 December 2019 9,143 1 View
Is it appropriate to have Person A as an Associate Editor of a submitted manuscript - where 1/4 of the total references are his/her own and who has been a co-author with the corresponding author...
14 December 2019 7,092 3 View
Dear all, There are lots of published work on sub-threshold operation and models of MOS devices (example: on Journal of Solid-State Circuits, IEEE). For a good and highly-credited one, could you...
08 December 2019 5,968 5 View
As I am not a native English speaker, normally, I am advised to use English editing services before submitting my manuscript to the journal. Recently, I got some bad experiences with the service I...
28 November 2019 7,039 11 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...
03 March 2021 2,870 3 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...
01 March 2021 1,189 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View