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Questions related from Khan Md Taufiqul Arif
The compound we have been using has been showing strong anti-inflammatory activities in dermal fibroblast cell, we believe that it might have shown the similar role in cornial epithelial cell....
08 December 2016 9,176 2 View
To get the best ROS generation (i am expecting 40-50% increase than that of control), how long my HUVEC should be starved and what would be the serum percentage? i am using EBM-2 (Lonza) without...
29 July 2016 2,228 4 View
In vitro, TNF- induces a very weak oxidative response by neutrophils but strongly enhances ROS release on exposure to a secondary stimulus, such as the bacterial peptide fMLF. is it capable of...
04 July 2016 7,109 2 View
I am culturing HUVEC up to 80% confluency (EGM-2; Lonza), then let them serum starved (EBM-2; Lonza) for 24hr and treated with TNF-a (10ng/ml) for 30min. With DCFDA I didn't get that much of...
17 June 2016 4,400 5 View
Trying to measure protein concentration for Lucigenin assay. But even culturing at more than 80% confluency, after centrifugation and sonication getting only 330ug/ml of protein. Hard to maintain...
15 June 2016 1,343 2 View
I am not controlling any of these factors, but feeling a bit shaky. Do I need to maintain a certain wavelength and what would be the optimum temperature for that? Thanks in advance.
07 June 2016 2,830 4 View
difference in terms of total ROS and/or type of ROS, after TNF-a challenge
12 May 2016 2,437 5 View
i would like to test my compound in vitro. which cell line should I use to generate atherosclerosis? to prove my compound's (Kinase inhibitor) potency which assays would be rational ?
10 May 2016 1,732 3 View
HUVECs are being starved for 24 hr (in 96-well) and same volume to of DCFDA is being added directly. Do I need to replace the media before adding DCFDA? Can cytokines cause any effect on ROS...
11 April 2016 6,299 2 View
my drug doesn't last long (~1hr at best) in the blood stream, so need know how long the lps actually remains there to cause sepsis. I found that treating my drug (20mg/kg bw; i.p.) after 1hr of...
14 March 2016 3,984 16 View
Assay: Cytokine profile and Survival rate determination Animal Model: C57BL/6 mouse (6weeks) LPS: E. coli: O111:B4, O55:85, O127:B8, Salmonella enterica ser. enteritidis/typhimurium Dose: 15mg/kg...
11 February 2016 681 3 View
Need to know the concentration, dosage and preparation of LPS, I am going to test my drug whether it has any anti-sepsis action.
01 February 2016 5,712 3 View
I have found that a tetrameric form of Ang-I would do good but, what is the molecular weight of that particular state and to which company I should look for.
21 January 2016 3,296 2 View
Model: Balb/c mouse (male) Compund: CCl4 Methods: 1. s.c injection: 0.5ml/kg bw, thrice/week upto 4 weeks 2. i.p injection: 0.5ml/kg bw, twice/week upto 4 weeks 3. oral gavage: 0.5ml/kg bw,...
10 December 2015 8,539 10 View
I have been searching for SNPs in miRNA genes (formulated panel of 65) in a designated breast cancer population by iPLEX/MALDI-TOF-MS. But after that just got stuck into and couldn't go...
01 January 1970 3,698 1 View
Dear Tuan Zea Tan, My research is focused on association of miRNAs to the molecular subtypes of breast cancer and their clinical significance. I'd really appreciate if you could share the...
01 January 1970 3,845 2 View
