i have been using lucigenin (5uM; final conc.) preppared in Krebs-Ringer-HEPES (modified) buffer. My stock is 12mM (in acetic acid) and for working I prepared it as 40uM in the same buffer. But the volume was more than I required. There comes the point that, can I preserve this extra solution? if so, how? and can I reuse that solution?
Regards
Hi
Take the UV-VIs and a Fluorescence emision spectra of your stock. Compare with the previously obtained spectra. In prnciple you can use it as long as you are savingyou have stored in the dark, around 0 - 4 °C and before store you can also bubbling nitrogen or argon gas in the solution. I am sure this will be useful sonce the solution is buffered. Just take care with your measurements, you must record your blanks in order to ensure that your stock is not photodegradated.
Best!
You can sure to stability of lucigenin by measuring absorption and fluorescence spectra and comprise with preious data.do not use surfactant .
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