I am using both soluble collagen form (20 micro gram/mL, serum free DMEM, over night incubation)and the coating techniques (200micro gram/well, 4 hrs incubation).
Thanks Dr. Bateman, I have got it... actually I tried to make the collagen solution with DMEM instead 0.012N HCl, so that I could skip the PBS washing. But it seems alright with HCl...