In the case of neutrophils (1 × 105) researchers have stimulated cells with 25 nM PMA or 0·75 mM HOCl and incubated for 3 h at 37°C. These cells were seeded into BSA-coated [1% in PBS) . HOCl was necessary and sufficient reactive oxygen intermediates (ROIs), particularly hydrogen peroxide and neutrophil extracellular traps (NETs) NET release.