I am not controlling any of these factors, but feeling a bit shaky.
Do I need to maintain a certain wavelength and what would be the optimum temperature for that?
Thanks in advance.
Dear Khan Md Taufiqul Arif,
The attached file and following link describe the details of the required assay.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153160/
Hoping this will be helpful,
Rafik
Dear Rafik Karaman,
Thank you for the article. It solved the temperature issue.
Best,
Taufiq
If you are using lucigenin luminescence as your readout, the wavelength does not need to be controlled. One generally tries to collect all the light emitted by the sample, without regard to wavelength.
If you are using lucigenin fluorescence as your readout, the peak excitation wavelength is 455 nm and the peak emission wavelength is 505 nm.
Dear Adam,
Thank you very much. Now out of confusion.
Best,
Taufiq
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