i would like to test my compound in vitro. which cell line should I use to generate atherosclerosis?
to prove my compound's (Kinase inhibitor) potency which assays would be rational ?
Look into the paper that is referenced in this SciGine method. They seem to be using rabbit aortic smooth muscle cells.
However, the results on the website seem to indicate that an in vivo model is a significantly better choice when it comes to testing drugs to aid in the treatment of atherosclerosis. I've attached a rabbit model for you in case you're interested.
http://scigine.com/method.php?ID=184381
http://scigine.com/method.php?ID=54095
Dear Karthik,
Thank you. I'll eventually go for the in vivo model, (apoe-/- model with WTD). I am in need of an in vitro model with which I could get some idea first. That's why looking for a simple system that would resemblance the in vivo condition. I found that, cells isolated from atherosclerotic lesions of human aorta retain all the main properties of atherosclerosis in culture. Anyway, thank you again.
I appreciate your kind help.
Obviously, there is no good model for a complex process such as atherosclerosis in a cell culture system. However, you could test your compounds in isolated cells in culture or co-culture if you have a predicted mechanism of action. For instance, if you expected an effect on monocyte targeting, you could examine monocyte adhesion to the endothelium (preferably under flow) in response to an atherogenic stimulus such as oxLDL treatment. However, the model will be limited to the specific process you are trying to reproduce in culture.
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