29 Questions 28 Answers 0 Followers
Questions related from Justin Tan
Dear all, good day! I have a question regarding the use of NaOH for lysis of mammalian cells. Is it a common practice? Are there any publications which have used NaOH to perform cell lysis? If...
04 April 2019 5,400 4 View
Dear all, recently i did a scratch test migration assay on my test compounds incubated with my cell line of interest and it appeared that the cell line seem to exhibit signs of increased cell...
11 November 2018 442 5 View
Dear all, recently i am considering if i have been doing my MTT assay wrongly as i have been using my media (with 10% FBS) for my cell viability assay. I realised that the cells were dying at high...
11 November 2018 8,959 2 View
Dear all, recently i found some strange and uncanny sights in the wells of cells treated with my compound of interest. Apparently when i checked the water solubility of the compound of interest,...
10 October 2018 1,489 3 View
Dear all, Recently i have a really crazy and brilliant idea to determine if a compound would be able to increase / decrease the motility of a cell type towards another cell type. How should i go...
10 October 2018 3,002 5 View
Dear all, recently i started a cell viability study using presto-blue reagent for the testing. However, i dissolved the drug of interest in sterile water before adding into the wells (96 well...
05 May 2018 9,027 0 View
Dear all, i have recently discovered that one of my test compounds is able to promote intercellular aggregation (i.e. the cells are aggregating when treated with the compound). However, i can only...
05 May 2018 7,275 1 View
Dear all, Recently I ran qPCR for 3 different sets of treated samples and the trend of the mRNA seemed to exhibit an increasing trend with increase treatment for all the genes tested. However,...
11 November 2017 7,354 4 View
As above. Thank you :)
11 November 2017 9,318 1 View
11 November 2017 7,748 4 View
11 November 2017 6,294 0 View
As above and i'm not sure why do the cells not remain in 3D aggregates. I tried to mix 2 cell types together and they do not "stick". What are the possible reasons for this? Thank you.
11 November 2017 1,419 2 View
Dear all, i was confounded by a phenomenon i witnessed under my light microscope - that is, when i tried mixing two different cell types together, i started to observe dark aggregates forming over...
10 October 2017 2,715 5 View
Dear all, my question is as above for immunofluoroscence staining purposes. Thank you for your kind responses.
10 October 2017 6,230 2 View
Dear all, I am working on a co-culture model recently which involves adhering HaCAT to another cell line. Do you have any advice what are the protein coating/adhesives available that can i use to...
10 October 2017 2,277 1 View
Dear all, recently i have tried to optimise my cell culture model by adding growth factors to it. The question is, which concentrations should i try? Most literature have reported using about 10...
09 September 2017 2,335 4 View
Dear all, Recently i tried to stain some co-cultured cells and realised that both the co-cultured cells and the mono-cell type both showed the fluorescence - indicating the presence of the...
09 September 2017 8,894 11 View
Dear all, I have recently bought the LDH Cytotox-one homogeneous membrane integrity assay but it seems that my compound, which is coloured, seems to produce lower fluorescence signal in comparison...
06 June 2016 7,628 0 View
I would like to stain some cell lines for the following proteins with specific antibodies: 1) CD133 2) Versican I have tried searching online for protocols, but I could not find any, even on...
04 April 2016 9,041 3 View
As above. Thanks :)
03 March 2016 2,044 5 View
Dear all, for the case of MTT assay, does the volume of formazan crystal solubilising agent such as DMSO affect the optical density (abs) of the well?
03 March 2016 1,088 4 View
Dear all, I have been working to troubleshoot my LC-MS/MS/MS experiments for several months already. There seems to be multiple peaks of the same product ion observed on the LC-MS chromatogram but...
03 March 2016 7,699 15 View
I am running an assay requiring the enzyme 5a-reductase, which I will be using PC-3 cells has its source and hopefully after some protein/enzyme purification, I might be able to obtain some...
01 January 2016 8,453 2 View
Just curious because this seems like a common procedure but not sure why is this step necessary.
01 January 2016 5,901 4 View
Any suggestions would be helpful. Thanks and warmest regards.
01 January 2016 10,002 0 View
Dear all, I would like to isolate the nuclei from the cells because the enzyme i would like to run my assay with is found on the nuclear envelope of the cells. How should i do this separation...
01 January 2016 506 1 View
I am trying to isolate the enzyme 5a-reductase from my PC-3 (Prostate Cancer) cell line. Does anyone have any advice to give concerning this extraction? For your kind advice, please. Thank...
01 January 2016 1,754 6 View
I am doing some cell work which requires me to incubate some cells with a few compounds of interest. I have read from a few articles claiming to remove the complete DMEM (growth media) and adding...
12 December 2015 7,703 13 View
I am running a 5a-reductase inhibition study, but PC-3 cell lysates did not seem to produce DHT upon testosterone incubation, so what other cell lines could possibly present me with a rich source...
11 November 2015 1,007 3 View
