50 Questions 430 Answers 0 Followers
Questions related from Go J Yoshida
I have performed patient-derived xenograft model (PDX) using NOG mice. 3 out of 10 mice in which tumor has grown has been shown to be malignant B cell lymphoma positive for CD20. Several papers...
16 March 2019 1,602 0 View
Gain-of-functional mutant p53 exhibits a longer half-life period as compared with wild-type p53, which is why the significant accumulation of the mutant p53 in cancer cells is recognized in many...
28 April 2018 7,030 4 View
Besides high-resolution and ultra-high-field (7-tesla) magnetic resonance imaging (MRI),1 proton MR spectroscopy holds much promise for the diagnosis of multiple sclerosis (MS).2,3 1H-MR...
05 February 2018 3,287 5 View
There has been a growing concern about the significant shortage of the number of young physician-scientists, who can contribute to the development of basic medicine with the objective of...
30 January 2018 9,794 10 View
When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...
28 January 2018 3,307 2 View
I wonder whether or not high-dose administration of cyclophosphamide causes arrhythmia as well as other cardiopulmonary disorders such as congestive heart failure and pulmonary hypertension. I am...
24 January 2018 197 9 View
Infected aortic aneurysms are diagnosed on the basis of a positive bacterial blood culture, clinical evidence of inflammation, and morphologic findings on computed tomography (CT). However,...
24 January 2018 2,031 2 View
It has been demonstrated that that cells lacking TSC2 have low levels of autophagy under basal and cellular stress conditions such as hypoxia and hypo-nutrition. Using genetic and...
23 January 2018 2,100 3 View
We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...
21 January 2018 7,952 2 View
It has been shown that the treatment with ruxolitinib, which selectively blocks JAK1/2-dependent signaling pathways, resulted in a high response rate exceeding 80% among corticosteroid-refractory...
20 January 2018 1,989 1 View
The availability of magnetic resonance imaging (MRI) has led to an increase in the detection of abnormal brain findings even in cases when there are no clear neurological symptoms. When these...
16 January 2018 8,960 14 View
As described in the very recent case report by New England Journal of Medicine (Case 2-2017), the chelation therapy would increase the serum copper concentration and cause the worsening of...
20 January 2017 5,002 4 View
The emerging evidence suggests that the drawbacks of senescence are twofold. First, one might expect, senescence causes a loss of tissue repair capacity because of cell cycle arrest in progenitor...
19 January 2017 7,120 2 View
I wonder whether or not the ammonia concentration of Wilson's disease presenting as fulminant hepatic failure does not increase? According to the very recent literature by NEJM Case Record (Case...
19 January 2017 536 4 View
Myasthenia gravis (MG) is an antibody-mediated autoimmune disorder of the neuromuscular junction. Approximately 20% of patients with generalized MG are “seronegative (herein, negative for...
16 January 2017 464 6 View
DNA methylation and epigenetic inactivation of the O6-methylguanine methyltransferase (MGMT) gene induces MGMT deficiency, reducing the tumor cell's DNA repair capacity and increasing its...
24 December 2016 3,042 2 View
Immunecheckpoint inhibitors such as Pembrolizumab, nivolumab (anti-PD-L1 agents) and Ipilimumab (anti-CTLA4 antibody) can cause myocarditis as the side effect. The same T cell clone population is...
06 November 2016 2,677 5 View
There are numerous papers that when autophagy-lysosome pathway is disturbed and the accumulated autophagosomes without the fusion with the lysosome is observed, the cells undergo apoptosis pathway...
01 November 2016 3,540 9 View
In the brain of the cirrhosis patients, excessive amount of ammonia crosses the blood–brain barrier, where astrocytic glutamine synthetase converts ammonia and glutamate into glutamine, which in...
31 October 2016 3,208 4 View
The following case record did not mention the effect of lithium for the treatment of ADHD on the breast and lung cancer. Given that lithium inhibits GSK-3 beta, it is likely that long-term use of...
28 October 2016 1,860 1 View
As indicated in the Case Record 23-2016 (July 28 issue) published by NEJM, a 46-year-old male patient developed rapidly progressive somnolence 12-14 hours after proximal femoral replacement...
20 August 2016 8,908 5 View
It is surprising in the following editorial that monocytes promote extravasation, which is the rate-limiting step of the distant metastasis! What about neutrophils!? Do they promote or suppress...
27 July 2016 6,296 6 View
Thiazolidinedione (TZD) binds to nuclear transcriptional factor PPAR-gamma, thereby causing metabolic reprogramming. This drug for the treatment for insulin resistance in type-II DM has been...
14 July 2016 4,835 2 View
It has been reported that excessive inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha in the serum secreted from the tumor tissues are responsible for the development of weight loss up...
14 July 2016 2,801 3 View
As compared with erastin, the target molecules of which are xCT cystine/glutamate antiporter and VADC2/3, artesunate was not specific to mutant RAS-driven cancer cells. For instance, erastin...
26 May 2016 5,394 0 View
TERT promoter mutations, IDH mutations and 1p/19q co-depletion are surely promising markers to classify clinically heterogeneous cases of glioma. Among Grade-IV gliomas patients, however, Figure1...
15 January 2016 7,749 1 View
Rasopathy is mainly composed of the cardiofaciocutaneous syndrome, Costello’s syndrome, Noonan’s syndrome, and the multiple lentigines syndrome. In addition, Legius syndrome is also well-known due...
15 January 2016 1,830 3 View
Contradictory to the widely-accepted concept that sexual transmission of Ebola virus (EBOV) is quite rare after the official end of an outbreak(1), the following preliminary case report suggests a...
15 January 2016 5,517 0 View
Dr. Ksenya Shchors et al. have recently published an original article on the drug repositioning of tricyclic antidepressants and anticoagulant ticlopidine, both of which provokes the autophagic...
22 October 2015 460 6 View
I would like to know the pathological significance of enhanced expression of cyclin D1 in hairy cell leukemia (HCL) cells, but not expressed in HCL-like leukemias/lymphomas (HCL-variant and...
11 September 2015 4,740 1 View
It has recently been reported that Axin2-positive peri-central vein hepatocytes correspond to the stem/ progenitor cells of the uninjured liver tissue (Nature 2015. doi:10.1038/nature14863). Wnt...
08 August 2015 5,229 0 View
Zeb1 is antagonized with Rb which controls the epithelial cell-to-cell adhesion. According to several papers, depletion of Zeb1 results in the active transcription of CDH1, encoding E-cadherin....
06 August 2015 4,126 1 View
I am confused with the two kinds of cohort analyses composed of discovery cohort and validation cohort shown in Figure2 e-h in the attached file.According to ROC curves, both analysis tells that...
25 July 2015 9,404 6 View
It has been demonstrated that Notch negatively regulates the development of neuroendocrine tumor and small cell lung cancer since the master transcriptonal factors such as Hes1 and Hey1 inhibit...
24 July 2015 4,770 6 View
It has been reported that the significant decrease in hair follicle stem cell (HFSC) in the niche results in the formation of the cyst in the skin. However, I am quite confused how the cyst...
11 June 2015 2,570 3 View
Devic disease, also referred to as neuromyelitis optica or NMO, is known as an immunological disorder that is frequently misdiagnosed with multiple sclerosis (MS). Devic disease is often...
26 May 2015 9,838 2 View
I would like to perform Western blotting analysis of 3D-cultured cancer cells in collagen gel. In the case of Matrigel, Cell Recovery Solution (Corning) is very useful, but I do not know exactly...
12 May 2015 595 8 View
Perineural invasion is frequently observed in pancreatic cancers and they exhibit focal differentiation surrounding the peripheral nerves. Neighboring schwann cells lead to the differentiation of...
21 April 2015 5,462 2 View
We have previously identified that IR or DMBA genotoxic stress cause micro-sized cysts in the skin, and this phenomenon is significantly observed in p53 -/- mice. Both WT and p53 -/- mice exhibit...
23 March 2015 5,492 5 View
Recent experiments revealed that both IR stress and DMBA treatment increase the stem cell factor (SCF) expression level at the secondary hair germ and dermal papillae. However, IR stress causes...
18 March 2015 3,110 2 View
I have recently been interested in MITF and its interaction with tyrosine kinase receptor family and performed several experiments using melanoma cell lines and xenograft model. However, according...
27 February 2015 8,352 1 View
We have previously identified the decreased expression of HFSC markers such as Lhx2, ColXVII, CD34 and Sox2 in the aging mice. However, the number of HFSCs was not so different as compared with...
03 February 2015 950 11 View
Neurofibromatosis type-I (NF-1), also referred to as von Recklinghausen's disease is a developmental disorder caused by germline mutations of NF1 genes encoding inneurofibromin, a gene that is...
25 January 2015 8,615 2 View
I often have difficulty in immunostaining of the structure of 3-D cultured cells, often referred to as spheroids or organoids embedded in Matrigel. What kind of methods/ protocols do you usually...
21 January 2015 4,732 9 View
We are interested in the dynamics of quiescent and proliferative cancer stem cells (CSCs) when tumor tissue shows invasion. We have previously investigated into the expression level of CD44...
18 January 2015 5,516 3 View
Bovine brucellosis is a major disease of cattle characterized by abortion during the last trimester of gestation. During many years important pieces of research have been done looking for a better...
01 January 1970 1,690 1 View
Inhibition of autophagy has been widely explored as a potential therapeutic intervention for cancer. Different factors such as tumor origin, tumor stage and genetic background can define a tumor's...
01 January 1970 9,133 1 View
It is widely accepted that E-cadherin expression is suppressed in metastatic cancer cell. However, there are two possibilities in vivo underlying the down-regulation of E-cadherin; 1) Tumor cells...
01 January 1970 8,696 3 View
It has been reported that TSC2-null cells exhibit the significant down-regulation of epithelial markers including occludin, caludin, and E-cadherin with the stable expression of Snail, one of the...
01 January 1970 2,818 1 View
Multiple myeloma (MM) is a clonal bone marrow disease characterized by the neoplastic transformation of differentiated B cells. There are several papers focusing on cancer stem cells in MM....
01 January 1970 1,543 0 View
