It has been reported that TSC2-null cells exhibit the significant down-regulation of epithelial markers including occludin, caludin, and E-cadherin with the stable expression of Snail, one of the EMT-related transcriptional markers. On the other hand, TSC2-null cells do not express either N-cadherin or vimentin.

That is why I would like to have your opinion about whether TSC2 knockdown induces EMT.

Thank you in advance.