25 Questions 6 Answers 0 Followers
Questions related from Fa Ro
Can all unsaturated fatty acid be converted to eicosanoids?
14 February 2023 7,967 2 View
Eicosanoids are generated from fatty acids but I was wondering where this conversion takes place? In the cells or outside of the cells?
02 February 2023 6,016 1 View
I used LCMS to detect metabolites and after a PCA plot was generated, I noticed that there were some sample outliers. What could be the cause of this?
02 February 2023 6,068 3 View
I treated cells with a toxic compound and was trying to find the most suitable treatment concentration. After performing a cell viability test, I saw that the middle concentration reduced the cell...
13 January 2023 6,271 2 View
I read in papers that the (indirect) conversion of tyrosine to fumarate can take place in cells but I was wondering if this also takes place in the brain, specifically in neurons?
18 November 2022 7,965 1 View
Which one is the end product? If the glycolytic pathway is upregulated, there should be more pyruvate present. If lactate levels are increased in a cell, does that mean that the glycolytic pathway...
14 September 2022 6,407 6 View
So long-chain fatty acids are dependent on the carnitine shuttle to enter the mitochondria but I read that short chain fatty acids do not require carnitines. I was wondering how and where...
02 September 2022 8,460 1 View
I was wondering whether NAD+ (not nicotinamide riboside or nicotinamide mononucleotide) can enter cells when supplementing the medium of a neuronculture with NAD+
18 August 2022 8,796 1 View
I did an LDH assay and have six concentrations of drugs I treated my cells with and when I put the LDH assay data in a graph, I see a normal distribution pattern. The 3rd concentration of the drug...
23 June 2022 6,539 2 View
The majority of short chain fatty acids (SCFA) is synthesized in the large intestine by gut microbiota but I was wondering how short chain fatty acids are synthesized in neurons and in the brain.
07 June 2022 205 0 View
I was wondering what the relationship is between malonyl carnitine and malonyl CoA
03 June 2022 4,369 0 View
I see that many papers include analyzing calcium in their methods when the differentiation of iPSCs to neurons is taking place. Why is this necessary?
28 September 2021 7,225 2 View
So excess NADH would inhibit the enzymes that generate NADH from NAD. This means that the citric acid cycle will be halted. What are the consequences? Is there a relevant article about this?
20 September 2021 9,984 3 View
Is there a specific reason why splenocytes are used to isolate immunecells from?
22 June 2021 5,144 3 View
I was wondering whether CD8 T cells that are present in the spleen are also present in the para-aortic lymph nodes during atherosclerosis. I can not find any article that studies the specific...
14 June 2021 5,046 2 View
I was wondering how long you can store samples and organs or tissues of mice under freezing conditions. I would like to use mice in my experiment and I will harvest the aorta, specifically aortic...
10 June 2021 9,403 3 View
I see that there are ldlr and apo deficient (-/-) mice, but for for example granzyme B, they are called granzyme B KO mice. Is there a difference in -/- deficient mice or KO mice
04 June 2021 5,101 3 View
I was wondering whether it is possible to turn off the expression of a transcription factor that is expressed by CD8 T cells, and if yes, how?
04 June 2021 8,208 1 View
I would like to know about all the molecules that can be expressed by/in CD8 T cells
01 June 2021 3,732 3 View
Streptolysin O can be used to deliver fluorescent probes into living cells. However it is not very clear to me whether immune cells, T cells specifically can also be permeabilized with Streptolysin O.
19 May 2021 8,426 1 View
I want to visualize EOMES, a transcription factor, in CD8 T cells. I am planning to perform an immunofluorescence test and I was wondering what kind of stain or chemicals I can use to enable the...
18 May 2021 9,886 7 View
For an adoptive transfer would like to isolate CD8 T cells that express granzyme K and EOMES from the spleen. Is it possible to first isolate CD8 T cells with an isolation kit, followed by the use...
14 May 2021 8,098 3 View
I often see in articles that they carry out just an adoptive transfer experiment or just a depletion study, and I was wondering whether you can do both or you only have to do one of them? I also...
12 May 2021 5,798 1 View
I have been looking at all kinds of articles but it is not very clear for me. CD4+ and CD8+ T cells have different functions. However, they do both secrete IFNy. So is the function of IFNy...
03 May 2021 8,431 3 View
I want to know whether all fatty axids go through beta-oxidation or if there are only several types of fatty acids that undergo this process
01 January 1970 4,001 7 View
