Is it possible to turn off the expression of a transcription factor that is expressed by CD8 T cells?

04 June 2021 1 8K Report

I was wondering whether it is possible to turn off the expression of a transcription factor that is expressed by CD8 T cells, and if yes, how?

Prateek Bhatia

Yes it us possible. You can design a sgRNA and then use CRISPR can system to knock out either the promoter region or the main gene of the transcription factor employing NHEJ mechanism of cells which is error prone and induces INDELs

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