I was wondering how long you can store samples and organs or tissues of mice under freezing conditions. I would like to use mice in my experiment and I will harvest the aorta, specifically aortic endothelial cells. I was wondering whether it is possible to store it for 1 year..
If you plan on isolating the cells, you cannot freeze the tissue. The ice crystals will destroy the cell membranes.
If you have isolated cells, that you can freeze in liquid nitrogen preferably or keep even at -86C in cryovials by using cryoprotectant like 10% DMSO or others available cryoprotectants. That is common practice using cryopreservation of cells in labs. You need to use a simple cryopreservation protocol. In brief, you can freeze. Ask any cell culture lab, they can tell you how to freeze cells. You can keep for one year in -86C and if for more years, in Liquid nitrogen.
Yes, you can freeze mice tissue over a year, but is important to use cryoprotectants like Subhash C. Juneja mentioned, you can use DMSO in order to reduce osmotic stress and also it's important to know that the process of freezing has to be fast, so when you add the DMSO it doesn't affect the cells because of its toxicity
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