For an adoptive transfer would like to isolate CD8 T cells that express granzyme K and EOMES from the spleen. Is it possible to first isolate CD8 T cells with an isolation kit, followed by the use of antibodies for granzyme K and EOMES?
If you are using miltenyi or similar kits it should be ok. The beads with this isolation kit are so small that they exert subphyiological effect on positively selected cells
Since granzyme K and EOMES are your target protein, negative selection, just as Urs mentioned, is better than positive purification. Abs on the positive seletion beads will activate target cells via cross linkage, this will bias later target protein detection, especially EOMES.
If you're worried about antibodies sticking to your cells after isolation, I can recommend the Fab-TACS® system. Instead of high affinity antibodies, low affinity Fab fragments are used to positively select cells. They completely dissociate from the surface after isolation, so you don't have to worry about antibodies remaining on your cells. You can check out the products here:
https://www.iba-lifesciences.com/products/cell-selection-expansion/cell-isolation-staining/
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