Dr. Godfred A. Menezes

31 Questions 157 Answers 0 Followers

Questions related from Dr. Godfred A. Menezes

Is it right to have exactly same acronym (IJPBS) for 2 different journal names?

“International Journal of Pharma and Bio Sciences” (IJPBS)- ISSN 0975-6299. International Journal of Pharmacy and Biological Sciences (IJPBS)- ISSN 2230-7605

09 September 2015 966 5 View

What is your experience with 1xTAE buffer for PFGE electrophoresis?

experience with 1xTAE buffer for PFGE electrophoresis!

01 January 2015 1,810 2 View

Have you tried in-house preparation of the restriction buffer?

Have you tried/ succeeded in in-house preparation of the restriction buffer (For restriction digestion) when you run out of it !!!!

11 November 2014 9,169 1 View

Is anyone using the NEB Lambda ladder PFG Marker?

NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...

11 November 2014 7,711 12 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...

09 September 2014 10,076 4 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

09 September 2014 9,106 9 View

Do you agree that starting with concentrated/ more than required template in PCR is one of the reason leading to false negative/ no result?

False negatives in PCR

08 August 2014 1,385 0 View

What should be the minimum criteria to call a scientific journal as indexed? Please give your personal opinions, as considered in your universities?

Indexed journal!!

08 August 2014 9,277 1 View

Do we still stick to the idea of three room rule for PCR Laboratory: separate room for extraction; reaction set up; and cycler location?

PCR Laboratory!

08 August 2014 8,918 3 View

Are buffered solution at neutral pH better than water for resuspending the master stock PRIMERS?

Resuspending the master stock PRIMERS

08 August 2014 1,987 6 View

How do you like the concept of MAGGOT THERAPY IN WOUND HEALING? How about its future?

Medical Maggots™ (maggot therapy, maggot debridement therapy, MDT, biotherapy, biosurgery, biodebridement, larval therapy). Maggot therapy is the controlled, therapeutic use of live blow fly...

08 August 2014 1,155 4 View

Is this social media trend raising awareness of a little-known disease or just trivialising serious illness- amyotrophic lateral sclerosis through IBC

ALS ice bucket challenge!

08 August 2014 1,385 1 View

EtBr is a carcinogen- is it a myth?

Are there actual cases of cancer developed being exposed to EtBr!!? There are only cell culture based evidences of EtBr being mutagenic!! Nevertheless I would personally protect myself from it and...

08 August 2014 1,881 4 View

What is the best way to dissolve Sodium N-lauroylsarcosinate or Sarcosyl (for PFGE) in water?

To dissolve Sodium N-lauroylsarcosinate or Sarcosyl salt (for PFGE) in water- for Gram Negative lysis buffer!!!

08 August 2014 2,950 9 View

Which is a better phenotypic test to confirm co-producers of AmpC beta-lactamases and ESBL's?

I trust that it has been a problem with phenotypic test to confirm co-producers of AmpC beta-lactamases and ESBL. Is the PCR for AmpC's and PCR-SEQUENCING for ESBLs the only option?

08 August 2014 425 13 View

In the direct manual DNA extraction from bacterial cells were you amused to see at times DNA being only along with sediment at the bottom?

In the direct manual DNA extraction from bacterial cells DNA has to be in the supernatant, but were you amused to see at times DNA being only along with the sediment? For Gram negative bacteria...

08 August 2014 5,217 3 View

In temporary refrigerated cultures on plates, when you open the plate at times we see crawling maggots, have you come across this situation?

Temporary stocks of bacteria on plates in refrigerator, crawling maggots and few flies at times Have you come across this situation? In fact the maggots would streak for us even more across the...

08 August 2014 8,347 8 View

In a bacterial semisolid stock if there is fungal contamination, how would you separate out the bacteria from contaminant fungus?

Single bacterial semisolid stock (though not the preferred way of stocking) contamination with fungus.

08 August 2014 7,942 13 View

Have you ever come across flying snakes? How do they fly?

Flying snakes?

07 July 2014 7,895 3 View

Do you think one should avoid specifying the primer sequence in publications, when it is from a reference and not self designed?

Primer sequence in publications.

07 July 2014 746 4 View

What makes the touch-me-not plant's movements!!??

touch-me-not plant's movements!!

07 July 2014 1,562 1 View

How vining plants search for support?

Vining plants and support

07 July 2014 5,559 5 View

Do you propose or oppose the the use of antibiotics (antimicrobials against bacteria) during viral fever to stop the secondary bacterial infection?

Antibiotics during viral fever to stop the secondary bacterial infection!!!!

07 July 2014 1,102 9 View

In developing countries, in some pharmacies, boys aged hardly 16-18 prescribe antibiotics. Do you think it is commendable!!??

U must watch the boys in Pharmacies, who are aged hardly 16-18, prescribing antibiotics. We have 2 types of important customers, one who do not earn enough to pay physician fee and the 2nd type...

07 July 2014 9,120 2 View

Can beverages containing caffeine (such as red bull and bison) lower the risk of developing type 2 diabetes?

Caffeine may lower the risk of developing type 2 diabetes

07 July 2014 6,238 20 View

Among PCR based bacterial typing methods which have you found quite promising?

PCR based typing methods such as- Ribotyping, RAPD etc.... For Enterobacteriaceae members I found ERIC-PCR to be quite good. Many Institutes do not have facilities for PFGE and MLST. Some...

07 July 2014 6,602 1 View

Is effective pipetting one of the important errors of beginners performing PCR?

Is it mainly because the volume is very small and hardly seen while you dispense?

07 July 2014 1,880 18 View

Is greenish yellow sputum recommended to start antibiotics (antimicrobials against bacteria) on the third day of viral fever?

Greenish yellow sputum recommended to start antibiotics on the third day of viral fever!!! Are there any references with Microbiological backup?

07 July 2014 3,146 5 View

What is the meaning of philosophical approach to research?

philosophical approach to research!!!

07 July 2014 6,840 2 View

Is Dr. Sci (DSc or ScD) considered superior to PhD?

Individuals choosing to pursue doctorate level education must sometimes choose between programs that award a Doctor of Philosophy (Ph.D.) and those that award a Doctor of Science...

07 July 2014 2,709 3 View

Can you share the crucial key points in PFGE optimising?

Crucial key points in PFGE optimising.

07 July 2014 2,041 4 View