Is it right to have exactly same acronym (IJPBS) for 2 different journal names?

08 September 2015 860 5 View

What is your experience with 1xTAE buffer for PFGE electrophoresis?

experience with 1xTAE buffer for PFGE electrophoresis!

31 December 2014 1,713 2 View

Is anyone using the NEB Lambda ladder PFG Marker?

10 November 2014 7,623 12 View

Have you tried in-house preparation of the restriction buffer?

10 November 2014 9,064 1 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

08 September 2014 9,985 4 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

08 September 2014 9,023 9 View

Do we still stick to the idea of three room rule for PCR Laboratory: separate room for extraction; reaction set up; and cycler location?

PCR Laboratory!

07 August 2014 8,834 3 View

Is this social media trend raising awareness of a little-known disease or just trivialising serious illness- amyotrophic lateral sclerosis through IBC

ALS ice bucket challenge!

07 August 2014 1,299 1 View

In a bacterial semisolid stock if there is fungal contamination, how would you separate out the bacteria from contaminant fungus?

07 August 2014 7,855 13 View

In the direct manual DNA extraction from bacterial cells were you amused to see at times DNA being only along with sediment at the bottom?

In the direct manual DNA extraction from bacterial cells DNA has to be in the supernatant, but were you amused to see at times DNA being only along with the sediment? For Gram negative bacteria...

07 August 2014 5,111 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View