What is your experience with 1xTAE buffer for PFGE electrophoresis?

More Dr. Godfred A. Menezes's questions See All

Is it right to have exactly same acronym (IJPBS) for 2 different journal names?

“International Journal of Pharma and Bio Sciences” (IJPBS)- ISSN 0975-6299. International Journal of Pharmacy and Biological Sciences (IJPBS)- ISSN 2230-7605

08 September 2015 1,039 5 View

Have you tried in-house preparation of the restriction buffer?

Have you tried/ succeeded in in-house preparation of the restriction buffer (For restriction digestion) when you run out of it !!!!

10 November 2014 9,243 1 View

Is anyone using the NEB Lambda ladder PFG Marker?

NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...

10 November 2014 7,800 12 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

08 September 2014 9,159 9 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...

08 September 2014 10,138 4 View

Do we still stick to the idea of three room rule for PCR Laboratory: separate room for extraction; reaction set up; and cycler location?

PCR Laboratory!

07 August 2014 8,981 3 View

Is this social media trend raising awareness of a little-known disease or just trivialising serious illness- amyotrophic lateral sclerosis through IBC

ALS ice bucket challenge!

07 August 2014 1,440 1 View

In a bacterial semisolid stock if there is fungal contamination, how would you separate out the bacteria from contaminant fungus?

Single bacterial semisolid stock (though not the preferred way of stocking) contamination with fungus.

07 August 2014 8,017 13 View

Do you agree that starting with concentrated/ more than required template in PCR is one of the reason leading to false negative/ no result?

False negatives in PCR

07 August 2014 1,442 0 View

What should be the minimum criteria to call a scientific journal as indexed? Please give your personal opinions, as considered in your universities?

Indexed journal!!

07 August 2014 9,317 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View