False negatives in PCR
“International Journal of Pharma and Bio Sciences” (IJPBS)- ISSN 0975-6299. International Journal of Pharmacy and Biological Sciences (IJPBS)- ISSN 2230-7605
08 September 2015 1,039 5 View
experience with 1xTAE buffer for PFGE electrophoresis!
31 December 2014 1,864 2 View
Have you tried/ succeeded in in-house preparation of the restriction buffer (For restriction digestion) when you run out of it !!!!
10 November 2014 9,243 1 View
NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...
10 November 2014 7,800 12 View
Synergism along with antibiotics against antibiotic resistant bacteria!
08 September 2014 9,159 9 View
Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...
08 September 2014 10,138 4 View
PCR Laboratory!
07 August 2014 8,981 3 View
ALS ice bucket challenge!
07 August 2014 1,440 1 View
Single bacterial semisolid stock (though not the preferred way of stocking) contamination with fungus.
07 August 2014 8,017 13 View
Indexed journal!!
07 August 2014 9,317 1 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
