Have you tried/ succeeded in in-house preparation of the restriction buffer
(For restriction digestion) when you run out of it !!!!
The problem was particularly with the promega product for PFGE. Found other restriction enzymes with more of buffer accompanied.
“International Journal of Pharma and Bio Sciences” (IJPBS)- ISSN 0975-6299. International Journal of Pharmacy and Biological Sciences (IJPBS)- ISSN 2230-7605
08 September 2015 1,039 5 View
experience with 1xTAE buffer for PFGE electrophoresis!
31 December 2014 1,864 2 View
NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...
10 November 2014 7,800 12 View
Synergism along with antibiotics against antibiotic resistant bacteria!
08 September 2014 9,159 9 View
Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...
08 September 2014 10,138 4 View
PCR Laboratory!
07 August 2014 8,981 3 View
ALS ice bucket challenge!
07 August 2014 1,440 1 View
Single bacterial semisolid stock (though not the preferred way of stocking) contamination with fungus.
07 August 2014 8,017 13 View
False negatives in PCR
07 August 2014 1,442 0 View
Indexed journal!!
07 August 2014 9,317 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View