Is it right to have exactly same acronym (IJPBS) for 2 different journal names?

08 September 2015 860 5 View

What is your experience with 1xTAE buffer for PFGE electrophoresis?

experience with 1xTAE buffer for PFGE electrophoresis!

31 December 2014 1,713 2 View

Is anyone using the NEB Lambda ladder PFG Marker?

10 November 2014 7,623 12 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

08 September 2014 9,985 4 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

08 September 2014 9,023 9 View

Do we still stick to the idea of three room rule for PCR Laboratory: separate room for extraction; reaction set up; and cycler location?

PCR Laboratory!

07 August 2014 8,834 3 View

Is this social media trend raising awareness of a little-known disease or just trivialising serious illness- amyotrophic lateral sclerosis through IBC

ALS ice bucket challenge!

07 August 2014 1,299 1 View

In a bacterial semisolid stock if there is fungal contamination, how would you separate out the bacteria from contaminant fungus?

07 August 2014 7,855 13 View

In the direct manual DNA extraction from bacterial cells were you amused to see at times DNA being only along with sediment at the bottom?

In the direct manual DNA extraction from bacterial cells DNA has to be in the supernatant, but were you amused to see at times DNA being only along with the sediment? For Gram negative bacteria...

07 August 2014 5,111 3 View

Do you agree that starting with concentrated/ more than required template in PCR is one of the reason leading to false negative/ no result?

False negatives in PCR

07 August 2014 1,293 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View