Dan Med

14 Questions 14 Answers 0 Followers

Questions related from Dan Med

Is it normal after a co-culture values to mono-culture are almost similar?

I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I...

30 May 2018 3,767 3 View

How to calculate ethanol yield?

While performing a fermentation experiment using seaweed hydrolysate. I obtained an ethanol concentration of 0.6%, however I am uncertain of how much it is in g/L. I am really looking forward to...

30 March 2018 9,596 2 View

Does drying seaweed at 60 degrees destroy composition?

I am planning to use the seaweed for bioethanol production and I am just curious to find out whether it might affect the amount of sugars.

11 February 2018 7,673 6 View

Is there anyone very familiar with DNS method for reducing sugars?

I am working on an experiment using Ulva lactuca as a substrate. At first I place 5 grams in 100 ml 0.4 N HCl .After this I added cellulase the reducing sugars rose to about 34 mg/ml. After...

30 January 2018 1,611 4 View

Are there any method to co-culture a fast growing yeast and a slow growing yeast?

I have been struggling so far with my co-culture of Pichia stipitis BCRC 21777 and Kluyveromyces marxianus K-21. K-21 grows much faster than BCRC 21777, since I already co-cultured the two at the...

29 November 2017 1,542 7 View

Is it suitable for using 10 ul liters as an innoculation volume for spread plating?

I am trying to work on a CFU/OD calibration curve for Pichia stipitis BCRC 21777 and I need to do spread plating in the process,usually when I use 100 ul it takes too long and very tiring. I am...

02 November 2017 6,222 0 View

Is there a specific protocol that must be followed for co-culturing two yeast strains?

Right now i am working on an experiment using two yeast strains Pichia stipitis BCRC 21777 and Kluyveromyces marxianus K 21. I am planning to observe their fermentation capabilities using basic...

18 October 2017 790 3 View

How long does it take yeast to reach an OD of 0.8?

I'm using the yeast Pichia stipitis BCRC 21777 and im growing it in 5 ml YPD broth. However, its been about 30 hours and the OD 600 is about 0.5 nm. I am not sure if this is normal or maybe as a...

30 September 2017 6,806 3 View

Which yeast species are able to utilize both glucose and rhamnose for bioethanol production?

I am working on an experiment to produce bioethanol using seaweed hydrolysate as a substrate. However, there are two sugars present (Rhamnose and Glucose), the yeast strain I have is Kluyveromyces...

14 August 2017 2,220 7 View

Is just filtering L-Rhamnose monohydrate ; its reagent grade in 0.22 ul enough to make HPLC grade?

the sample I got was L-Rhamnose monohydrate but its reagent grade. Is just filtering enough to make it HPLC grade? Or are there other methods. I am going to use the rhamnose in order to make a...

10 August 2017 6,925 4 View

Can cultivating yeast in one medium and then spread plating them on a different medium (agar) have a bad influence on results?

I am going to perform an experiment using Very high gravity ethanol fermentation medium to monitor the residual glucose, cell number and ethanol production over a hourly period. I performed the...

24 April 2017 7,304 2 View

What is the glucose ranges that can be measured using the DNS method of reducing sugars?

I used two concentrations of glucose 0-1 mg/ml and 0-25 mg ml. The problem is that the higher concentrations are similar in color and the lower concentrations a clear difference can be noticed....

09 April 2017 8,737 3 View

I need to create a glucose standard curve using the DNS method. My question is how to prepare a standard glucose solution?

I am not sure whether the standard can be prepared by using the glucose powder. Really looking forward to your response.

23 March 2017 3,869 3 View

What is the best procedure to make a growth curve from the yeast kluyveromyces marxianus from the -80 degree C freezer?

I first retrieved the yeast from the -80 degree Celsius freezer and then place it onto YM agar plate and then stored at 30 degree Celsius. Next day I took a colony from that plate and added it to...

09 February 2017 2,943 2 View