16 Questions 16 Answers 0 Followers
Questions related from Dan Med
I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I...
30 May 2018 3,836 3 View
While performing a fermentation experiment using seaweed hydrolysate. I obtained an ethanol concentration of 0.6%, however I am uncertain of how much it is in g/L. I am really looking forward to...
30 March 2018 9,650 2 View
I am planning to use the seaweed for bioethanol production and I am just curious to find out whether it might affect the amount of sugars.
11 February 2018 7,732 6 View
I am working on an experiment using Ulva lactuca as a substrate. At first I place 5 grams in 100 ml 0.4 N HCl .After this I added cellulase the reducing sugars rose to about 34 mg/ml. After...
30 January 2018 1,658 4 View
I have been struggling so far with my co-culture of Pichia stipitis BCRC 21777 and Kluyveromyces marxianus K-21. K-21 grows much faster than BCRC 21777, since I already co-cultured the two at the...
29 November 2017 1,623 7 View
I am trying to work on a CFU/OD calibration curve for Pichia stipitis BCRC 21777 and I need to do spread plating in the process,usually when I use 100 ul it takes too long and very tiring. I am...
02 November 2017 6,263 0 View
Right now i am working on an experiment using two yeast strains Pichia stipitis BCRC 21777 and Kluyveromyces marxianus K 21. I am planning to observe their fermentation capabilities using basic...
18 October 2017 838 3 View
I'm using the yeast Pichia stipitis BCRC 21777 and im growing it in 5 ml YPD broth. However, its been about 30 hours and the OD 600 is about 0.5 nm. I am not sure if this is normal or maybe as a...
30 September 2017 6,869 3 View
I am working on an experiment to produce bioethanol using seaweed hydrolysate as a substrate. However, there are two sugars present (Rhamnose and Glucose), the yeast strain I have is Kluyveromyces...
14 August 2017 2,267 7 View
the sample I got was L-Rhamnose monohydrate but its reagent grade. Is just filtering enough to make it HPLC grade? Or are there other methods. I am going to use the rhamnose in order to make a...
10 August 2017 6,992 4 View
well i am working with yeast to produce ethanol but i am a little confused what 0-5% ethanol is i mean how many grams per litre is it equal to and basically using this information how may i...
01 June 2017 6,701 3 View
I am going to perform an experiment using Very high gravity ethanol fermentation medium to monitor the residual glucose, cell number and ethanol production over a hourly period. I performed the...
24 April 2017 7,343 2 View
I used two concentrations of glucose 0-1 mg/ml and 0-25 mg ml. The problem is that the higher concentrations are similar in color and the lower concentrations a clear difference can be noticed....
09 April 2017 8,998 3 View
i prepared my reagent as follows 30 g potassium sodium tartrate, 1.6 g NaOH, and 1 g 3,5-Dinitro-2-hydroxybenzoic acid. I filled it up to 100ml. I prepared my glucose standard solution as...
27 March 2017 3,575 5 View
I am not sure whether the standard can be prepared by using the glucose powder. Really looking forward to your response.
23 March 2017 3,906 3 View
I first retrieved the yeast from the -80 degree Celsius freezer and then place it onto YM agar plate and then stored at 30 degree Celsius. Next day I took a colony from that plate and added it to...
09 February 2017 2,985 2 View
