I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I performed Anova analysis using those values it was found insignificant. However, I always believed the value should be significant which shows the success of the co-culture but I am just a bit puzzled just to wonder whether was my co-culture really successful or not. Really looking forward to your expert opinions.
If you have conducted ANOVA correctly, you should rely on the outcome. Did you conduct experiments in triplicates? Is there any incompatibilities between the microbes? If I remember correctly, P. stipitis is susceptible to catabolite repression, and exhibits low ethanol/glucose tolerance we well.
I don't know about the microbiology part, however from the information above it seems that ANOVA was conducted incorrectly. ANOVA just shows the significance of the difference between two samples and the null hypothesis. The accuracy depends on how many repeats you conducted (at least triplicates) and what is variance between them and the experiments.
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