I need to create a glucose standard curve using the DNS method. My question is how to prepare a standard glucose solution?

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Ivan Shnypir

You can prepare the stock solution dissolvin crystalline glucose povder (250mg/100ml) then dilute it in required ratios (1:10, 1:4 ...).

George Lima Marques

You can prepare the stock solution as demonstrated in this standard method: http://ainfo.cnptia.embrapa.br/digital/bitstream/item/103342/1/BPD13017.pdf

Hossein Saffari

Based on amount of reducing sugar in samples You can prepare the stock solution from crystalline glucose powder (1000mg/1000ml) and then dilute it in different ratio(1:2,1:5, 1:10,...)