I am working on an experiment to produce bioethanol using seaweed hydrolysate as a substrate. However, there are two sugars present (Rhamnose and Glucose), the yeast strain I have is Kluyveromyces marxianus K-21. This yeast strain is able to utilize glucose, however, for Rhamnose I am not too sure since I have not carried out the experiment but on some studies it is not able to completely utilize fully Rhamnose. I have searched journals and papers about strains of yeast that are able to utilize both, such as Pichia stipitis and Candida shehatae but I am not sure specifically which strain is able to utilize both or just be able to utilize Rhamnose. I am really looking forward to your expert opinions. Thank you!
Hello Dan.
Firstly, Glucose and Rhamnose are different kind of sugars. Glucose is an hexose, and Rhamnose is methyl-pentose or a 6-deoxy-hexose.
As far as I know, "natural" yeast strains usually have one only metabolic pathway. For instance, the majority of Saccharomyces cerevisiae strains are able to use hexoses and produce ethanol. However, if you need to utilize both compounds (hexoses and deoxy-hexoses) it is likely that you will need a genetically modified microorganism (capable to ferment both) or perhaps try a cofermentation process (where you use two microorganisms in order to consume both Glucose and Rhamnose).
Sadly, I am not sure of what kind of microorganisms can ferment Rhamnose.
Sadly, I am not sure, but Pablo García del Río is right.
You can still use Saccharomyces cerevisiae.
Good luck
Dan Med - you can check with DSMZ (microbial culture collection in Germany) or The National Collection of Yeast Cultures (UK).
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