I am going to perform an experiment using Very high gravity ethanol fermentation medium to monitor the residual glucose, cell number and ethanol production over a hourly period. I performed the experiment before, however, the yeast did not grow well on the agar medium that I used ( YM agar), as a result of this, I am wondering if when also culturing the yeast they should also be spread on Very high gravity ethanol fermentation agar medium. If so, how much agar should be applied?
The basal fermentation medium contained 40 g of glucose, 5 g of yeast extract, 4 g of (NH4)2SO4, 0.3 g of MgSO47H2O, 0.3 g of CaCl2H2O and 1 g of KH2PO4 per liter of deionized water.
Looking forward to your response.
Thank You.
I apologize the medium that I will use is basal fermentation medium consisting of 40 g glucose, 5 g yeast extract, 4 g (NH4)2SO4, 0.3 g MgSO4 7 H20, 0.3 g CaCl2H20 and 1 g KH2PO4 per litre. I want to monitor reducing sugars, ethanol, and cell numbers. The problem is I don't know what agar I can do spread plating on. Should I just add agar to the basal fermentation broth? and if so what amount?
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