the sample I got was L-Rhamnose monohydrate but its reagent grade. Is just filtering enough to make it HPLC grade? Or are there other methods. I am going to use the rhamnose in order to make a standard curve. Really hope to hear your feedback.
a single filtration is not able to make a L-Rhamnose monohydrate reagent grade become a HPLC grade. HPLC grade reagents are generally controlled for high assay and low residues and water.
Usually an HPLC grade is 99% purity since even 95% can have hundreds of organic impurity peaks similar to your molecule of interest. I doubt very much simple filtration would be adequate.
Thank you guys for your kind responses. Are there methods that I may use to determine rhamnose concentration using reagent grade. Are TLC and Orcinol Methods good choices?
I see thank you, I really appreciate your help. HPLC -RID is available in my lab, however, an HPLC grade of L-Rhamnose isn't and I have already checked sites like sigma. All that is a available was reagent grade. This why I thought TLC would be a better choice.