I had worked following methods to isolate fungi
Is there any methods other than this? Please suggest a good articles regarding these methods.
The only thinks that can propose - please try to find Prof. J. Zak from Texas USA manuscripts or get in contact with him
I had refered some literature about Fungal DNA extraction. I had seen that CTAB , Phenol chloroform , Kit methods are commonly used ones. So which will best one for extraction of DNA from Marine...
22 October 2015 5,310 2 View
While transfering germinating spores into pda instead of my desired fungi other fastidious fungi and bacteria is inhibiting the growth of desired fungi. How I can overcome this contamination. How...
18 June 2015 5,897 12 View
Currently I am working on single spore isolation method. some spores are germinating and some are non germinating.When i transfered the germinating spores into PDA they are contaminated with...
17 June 2015 9,170 13 View
i have searched protocols for the estimation of a salinity of a sea water.but i need to know the widely used method please refer me good method
25 May 2015 6,788 5 View
It is very difficult to get Fungal Extract from many fungal cultures. I need to know whether any other methods to perform Antimicrobial microbial activity without extracting fungal extract. we can...
02 April 2015 7,559 13 View
I studied that ethyl acetate, hexane and methanol are best for extraction process. Are there any methods for identification of compounds apart from GCMS,HPLC,NMR? Can anyone suggest some good...
08 March 2015 5,663 5 View
I tried to induce sporulation by using sterile match sticks but thy are not sporulating Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro
03 March 2015 1,455 5 View
The literature suggests that we have to add 2% SDS and triflouroacetic acid or formic acid can be used for extraction of hydrophobins. Is there any other solvent which can be used for this...
03 March 2015 8,032 2 View
I had studied that internal transcribed spacer (ITS), Isolation of DNA and PCR amplification and DNA sequencing and BLAST and phylogenetic analysis are used. Can anyone suggest articles about...
03 March 2015 5,416 8 View
I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes
03 March 2015 8,031 6 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View